Transcriptional regulation
I. DNA Binding proteins - review DNA-protein struct.
methods to detect DNA binding proteins
gel retardation, DNAseI footprinting
affinity chromotography, expression screening
II. DNA Binding proteins - families
alpha helix - helix-turn-helix, Zinc finger, Pou
basic domain - leucine zipper
beta sheet
ribbon-helix-ribbon, TBP
III. RNA polymerases
polymerase/promoter types
transcription initiation complexes
bacterial
eukaryotic
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