Messenger RNA 3' End Formation in Plants
The major focus of this laboratory over the years has been the study of RNA polyadenylation in plants. Early work centered on the structure of plant polyadenylation signals. These studies led to a model for the plant polyadenylation signal that has been corroborated by more recent bioinformatic analyses, and established a foundation for biochemical and genetic studies that continue to this day.
A digression from these ongoing biochemical studies involved a characterization of a chloroplast-localized poly(A) polymerase. This area of study is no longer being actively pursued in the laboratory.
At the present, research efforts involve a combined biochemical, molecular,
and genetic characterization
of the nuclear polyadenylation apparatus in plants. The long-term goals
of these efforts are to characterize the means by which mRNAs are polyadenylated
in plants, and to understand the interplay between cellular signaling and mRNA
3' end formation.
Hunt
AG, Chu NM, Odell JT, Nagy F, Chua N-H. 1987. Plant cells do not properly
recognize animal gene polyadenylation signals. Plant Mol. Biol. 8: 23-35
Hunt AG. 1988. Identification and characterization of cryptic polyadenylation
sites in the 3' region of a pea ribulose-1,5-bisphosphate carboxylase small
subunit gene. DNA 7: 329-336.
Hunt
AG, MacDonald M. 1989. Deletion analysis of the polyadenylation signal of
a pea ribulose-1,5-bisphosphate carboxylase small subunit gene. Plant Mol. Biol.
13: 125-138.
Mogen
B, Graybosch R, MacDonald M, Hunt AG. 1990. Upstream sequences other than
AAUAAA are required for efficient mRNA 3' end formation in plants. Plant Cell
2: 1261-1272.
MacDonald
MH, Mogen B, Hunt AG. 1991. Characterization of the polyadenylation signal
of the T-DNA-encoded octopine synthase gene. Nuc. Acids Res. 19: 5575-5581.
Hunt
AG, Mogen BD, Chu NM, Chua N-H 1991. The SV40 small t intron is accurately
and efficiently spliced in tobacco cells. Plant Mol. Biol. 16: 375-380.
Yang J,
Hunt AG. 1992.. Purification and characterization of a 70 kD polyadenylate-binding
protein from pea (Pisum sativum). Plant Physiol. 98: 1115-1120.
Mogen
BD, MacDonald MH, Leggewie G, Hunt AG. 1992.. Several distinct types of
sequence elements are required for efficient mRNA 3' end formation in a pea
rbcS gene. Mol. Cell. Biol. 12: 5406-5414.
Yang J, Hunt AG. 1994. Immunological
characterization of plant polyadenylate-binding proteins. Plant Sci. 99: 161-170.
Hunt AG. 1994 Messenger RNA 3' end formation in plants. Ann. Rev. Plant Physiol. Plant Mol. Biol. 45: 47-60.
Li
Q, Hunt AG. 1995. A near upstream element in a plant polyadenylation signal
consists of more than six bases. Plant Mol. Biol. 28: 927-934.
Das Gupta J, Li Q, Thomson AB,
Hunt AG. 1995. Characterization of a novel plant polyadenylate polymerase.
Plant Sci.110: 215-226.
Li Q, Das Gupta J,
Hunt AG. 1996. A plant poly(A) polymerase requires a novel RNA binding protein
for activity. Journal of Biological Chemistry 271: 19831-19835.
LiQ, Hunt AG.
1997. The polyadenylation of RNA in plants. Plant Physiology 115: 321-325.
Li Q, Das Gupta J,
Hunt AG. 1998. Polynucleotide phosphorylase is a component of a novel plant
poly(A) polymerase. Journal of Biological Chemistry 273: 17539-17543.
Das
Gupta J, Li Q, Thomson AB, Hunt AG. 1998. Characterization of cDNAs encoding
a novel plant poly(A) polymerase. Plant Molecular Biology 37: 729-734.
Hunt AG, Meeks LR, Forbes
KP, Das Gupta, Mogen BD. 2000. Nuclear and chloroplast poly(A) polymerases
from plants share a novel biochemical property. Biochemical and Biophysical
Research Communications 272: 174–181.
Dattaroy T, Hunt AG. 2002. Polyadenylation of RNAs associated with a nuclear phosphorolytic nuclease complex from plants. Journal of Plant Biochemistry and Biotechnology 11: 21-25.
Elliott BJ, Dattaroy T, Meeks-Midkiff LR, Forbes KP, Hunt AG. 2003. An interaction between an Arabidopsis poly(A) polymerase and a homologue of the 100 kDa subunit of CPSF. Plant Mol. Biol. 51:373-84
Addepalli B, Meeks LR, Forbes KP, Hunt AG. 2004. Novel alternative splicing of mRNAs encoding poly(A) polymerases in Arabidopsis. Biochimica Biophysica Acta 1679: 117-128.
Forbes KP, Addepalli B, Hunt AG. 2006. An Arabidopsis Fip1 homologue interacts with RNA and provides conceptual links with a number of other polyadenylation factor subunits. J. Biol. Chem. 281: 176-186.
Delaney KJ, Xu R, Zhang J, Yun K-Y, Li QQ, Falcone DF, Hunt AG. 2006. Calmodulin interacts with and regulates the RNA-binding activity of an Arabidopsis polyadenylation factor subunit. Plant Physiol. 140: 1507-1521.
Addepalli B, Hunt AG. 2007. A novel endonuclease activity associated with the
Arabidopsis ortholog of the 30 kD subunit of cleavage and polyadenylation specificity
factor. Nucleic Acids Research, in press.
The Plant Poly(A) Signal
Database - an unpublished graphical representation of the base composition
near known plant polyadenylation sites